Gene Technology

Aim

The course will give fundamental knowledge and experimental skills in the following areas of Molecular Biology: Site-specific mutagenesis, gene cloning and PCR After studies well learned the student will have proficiency to:

• Understand basic laboratory techniques in molecular biology techniques to perform site-directed mutagenesis, amplification of DNA fragments using PCR and cloning of DNA fragments
• Describe different types of vectors for cloning and expression of eukaryotic genes in a prokaryotic system
• Theoretical knowledge of DNA sequencing
• Theoretical and practical knowledge of techniques for purification of plasmid DNA
• Describe the function and applications of different enzymes involved in gene cloning experiments

Content

Purifications of DNA, manipulation of DNA, cloning of genes into E.coli vectors, control of gene expression, differences in prokaryotic and eukaryotic genes, mutation analysis, DNA-sequencing.