Analytical Chemistry

Aim

The aim of the course is to give fundamental theoretical, practical and instrumental knowledge in the field of atomic and molecular spectrometry, and analytical separation techniques. After completing this course the student should be able to:

• Give an account of basic concepts within the area of atomic and molecular spectrometry, and analytical separation techniques (chromatography and capillary electrophoresis).
• Describe the principles and construction of instruments used for spectrometric analyses and analytical separations.
• Explain the chemical principles of spectrometric analyses and analytical separations.
• Interpret and qualitatively and quantitatively evaluate data obtained from spectrometric analyses and analytical separations.

Content

Theory of chromatographic separation. Gas chromatography (GC) including sample injection, separation and detection. High performance liquid chromatographic (HPLC) methods such as normal phase and reverse phase HPLC, size-exclusion chromatography and ion chromatography. Capillary electrophoresis. Mass spectrometry (MS), manual and computer-based interpretation of mass spectra, GC-MS and HPLC-MS. Molecular ultraviolet/visible absorption spectroscopy and molecular fluorescence.